The ability to assess the impact of small molecule drugs on cellular health is a powerful tool in drug discovery and development. The toolbox for measuring cellular health includes diverse reagents and readouts that are amenable to automation and high-throughput applications. Several classes of reagents, including tetrazolium salts, resazurin-based reagents, and ATP-dependent luciferase
enzymes, are common tools to evaluate cell viability and cytotoxicity by informing on distinct markers and mechanisms of cellular health. Several iterations of each reagent are available, differing in assay sensitivity, throughput, intrinsic toxicity, and overall data quality.

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