Lysine demethylases (KDMs) play a central role in epigenetic regulation. There are 27 KDMs separated into two classes that function to remove methyl groups from methylated lysine residues on histones, thereby regulating gene expression. Lysine demethylase 1 is a flavin adenine dinucleotide dependent monoamine oxidase that removes mono-/di-methyl groups. The Jumonji C-terminal domain (JmjC) family requires iron (II) and 2-oxoglutarate to remove mono-, di- and tri-methylated states. KDMs are now emerging as a valuable target in many therapeutic areas including cancer, neurological and genetic disorders.
What We Offer
SAMDI Tech offers a plug-and-play format for quantitative analysis of demethylase activity using native substrates. Native peptide and protein substrates can be captured and purified onto SAMDI 384 or 1536 biochip arrays for analysis by mass spectrometry. No need for cumbersome fluorescent-labeled substrates that can interfere with activity and lead to increase false positive rates. SAMDI has the capability to monitor all methylated states simultaneously in a single assay.