Lysine Methyltransferases are enzymes that play a critical role in epigenetic control of gene expression by facilitating transfer of one, two or three methyl groups to specific nucleophilic sites on proteins and nucleic acids. Methyltransferases fall into two major families—protein lysine methyltransferases (KMTs) and protein arginine methyltransferases (PRMT). Each family contains an evolutionarily conserved catalytic SET domain, which transfers methyl groups from S-Adenosyl methionine (SAM) to the target residue. Aberrant expression of HMTs has been detected in multiple myeloma and various genetic diseases making it a potential therapeutic target.
What We Offer
SAMDI Tech offers a plug-and-play format for quantitative analysis of methyltransferase activity using native substrates. Native peptide and protein substrates can be captured and purified onto SAMDI 384 or 1536 biochip arrays for analysis by mass spectrometry. No need for cumbersome fluorescent-labeled substrates that can interfere with activity and lead to increase false positive rates. SAMDI has the capability to monitor all methylated states simultaneously in a single assay.