Phosphatases are enzymes that catalyze the removal of phosphate groups from distinct substrates. They can be broadly characterized into classes defined by their target substrate, including protein (serine/threonine and tyrosine), acid, and alkaline. Phosphatases are key players in regulating cell behavior including adhesion, motility, and proliferation, and their implication in several pathologies including cancer and autoimmune disease highlights their potential value as therapeutic targets.
What we offer
SAMDI Tech offers a plug-and-play format for quantitative analysis of phosphatase activity using native substrates. Analytes including peptides, oligonucleotides, lipids, and small molecules can be captured and purified onto SAMDI 384 or 1536 biochip arrays for analysis by mass spectrometry. The SAMDI assay overcomes the limitations of label-based phosphatase assays by distinguishing their distinct activities and enables detection of multiple dephosphorylation events and multiplex assay formats.