Label-Free Phosphatase Assays
Phosphatases are enzymes that catalyze the removal of phosphate groups from distinct substrates. They can be broadly characterized into classes…
Label-Free Kinase Assays
Kinases have long been implicated in several pathologies including cancer. Kinases are broadly classified based on their target substrate, such…
Traceless Immobilization of Analytes for High Throughput Experiments with SAMDI Mass Spectrometry
Abstract: Label-free assays, and particularly those based on the combination of mass spectroscopy with surface chemistries, enable high-throughput experiments of…
Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities
Design of Glycosylation Sites by Rapid Synthesis and Analysis of Glycosyltransferases
Identification of small molecule noncovalent binders utilizing SAMDI technology
Abstract: In recent years, the ability to unambiguously identify complex mixtures of analytes with high accuracy and resolving power in…
Label-Free Demethylase Assays
Lysine demethylases (KDMs) play a central role in epigenetic regulation. There are 27 KDMs separated into two classes that function…
Label-Free Methyltransferase Assays
Lysine Methyltransferases are enzymes that play a critical role in epigenetic control of gene expression by facilitating transfer of one,…
Discovery of SIRT3 Inhibitors Using SAMDI Mass Spectrometry
HTS of Small Molecule Libraries using SAMDI
High-throughput screening is a common strategy used to identify compounds that modulate biochemical activities, but many approaches depend on cumbersome…