Traceless Immobilization of Analytes for High Throughput Experiments with SAMDI Mass Spectrometry
Abstract: Label-free assays, and particularly those based on the combination of mass spectroscopy with surface chemistries, enable high-throughput experiments of…
Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities
Design of Glycosylation Sites by Rapid Synthesis and Analysis of Glycosyltransferases
The SAMDI assay: a label-free, high-throughput platform for small molecule drug discovery using mass spectrometry
Label-Free Demethylase Assays
Lysine demethylases (KDMs) play a central role in epigenetic regulation. There are 27 KDMs separated into two classes that function…
Label-Free Methyltransferase Assays
Lysine Methyltransferases are enzymes that play a critical role in epigenetic control of gene expression by facilitating transfer of one,…
Label-Free Arginase Assays
Arginase catalyzes the hydrolysis of L-arginine to L-ornithine and urea. Ornithine serves as the biosynthetic precursor of proline, a critical…
Label-Free Protease Assays
Proteases are involved in protein processing, regulation of protein function, apoptosis, viral pathogenesis, digestion, photosynthesis, and numerous other essential processes….
Discovery of SIRT3 Inhibitors Using SAMDI Mass Spectrometry
HTS of Small Molecule Libraries using SAMDI
High-throughput screening is a common strategy used to identify compounds that modulate biochemical activities, but many approaches depend on cumbersome…